NSJ Bioreagents

Kallikrein-2 Antibody / KLK2

Product Code:
 
NSJ-RQ4132
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the KLK2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 2
IHC testing of FFPE human prostate cancer tissue with KLK2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
2 / 2
Western blot testing of human 1) MCF7, 2) COLO320, 3) SK-OV-3 and 4) HepG2 cell lysate with KLK2 antibody at 0.5ug/ml. Predicted molecular weight ~17-29 kDa (multiple isoforms).
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IHC testing of FFPE human prostate cancer tissue with KLK2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of human 1) MCF7, 2) COLO320, 3) SK-OV-3 and 4) HepG2 cell lysate with KLK2 antibody at 0.5ug/ml. Predicted molecular weight ~17-29 kDa (multiple isoforms).

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CodeSizePrice
NSJ-RQ4132-100ug100 ug£535.00
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the KLK2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
KLK2 (KALLIKREIN 2), also called GLANDULAR or PROSTATIC, is a protein that in humans is encoded by the KLK2 gene, and is particularly associated with prostatic tissue. The KLK2 is a member of glandular kallikrein gene family that comprises 25 to 30 highly homologous genes that encode specific proteases involved in the processing of biologically active peptides. The KLK2 gene is mapped to 19q13.33.And the KLK2 gene contains 5 exons. An alternative KLK2 transcript, which they call KLK2-linked molecule (KLM), that arises from the use of an alternate donor site within intron 1.KLM shares only the N-terminal 15-amino acid signal peptide with the original KLK2 protein; the mature proteins display no similarity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids I25-P261 was used as the immunogen for the KLK2 antibody.
Limitation:
This KLK2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P20151