NSJ Bioreagents

MAPK8/9 Antibody

Product Code:
 
NSJ-R30478
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MAPK8/9 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of MAPK8/9 antibody and Lane 1: rat brain; 2: rat thymus; 3: MCF-7; 4: HeLa; 5: Jurkat; 6: MM231; 7: CEM cell lysate
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IHC-P: MAPK8/9 antibody testing of rat intestine tissue
3 / 3
IHC testing of FFPE human intestinal cancer with MAPK8/9 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of MAPK8/9 antibody and Lane 1: rat brain; 2: rat thymus; 3: MCF-7; 4: HeLa; 5: Jurkat; 6: MM231; 7: CEM cell lysate
IHC-P: MAPK8/9 antibody testing of rat intestine tissue
IHC testing of FFPE human intestinal cancer with MAPK8/9 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

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CodeSizePrice
NSJ-R30478-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MAPK8/9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mitogen-activated protein kinase 8 is an enzyme that in humans is encoded by the MAPK8 gene. The protein encoded by this gene is a member of the MAP kinase family. The International Radiation Hybrid Mapping Consortium mapped the MAPK8 gene to chromosome 10. The single long open reading frame encodes a putative protein kinase with a predicted mass of 44.2 kD. This kinase play a key role in T cell proliferation, apoptosis and differentiation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human MAPK8/9 (EREHTIEEWKELIYKEVMDL) was used as the immunogen for this MAPK8/9 antibody (100% homologous in human, mouse and rat).
Limitation:
This MAPK8/9 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P45983