NSJ Bioreagents

MPI Antibody / Mannose Phosphate Isomerase

Product Code:
 
NSJ-R32582
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the MPI antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
Western blot testing of 1) rat testis and 2) human HeLa lysate with MPI antibody at 0.5ug/ml. Predicted molecular weight ~47 kDa.
2 / 7
IHC staining of FFPE human intestinal cancer with MPI antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 7
IHC staining of FFPE human lung cancer with MPI antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 7
IHC staining of FFPE rat brain with MPI antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 7
Western blot testing of 1) rat ovary and 2) mouse lung lysate with MPI antibody at 0.5ug/ml. Predicted molecular weight ~47 kDa.
6 / 7
Flow cytometry testing of human A549 cells with MPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPI antibody.
7 / 7
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human HEK293, 4) rat brain, 5) rat lung, 6) rat heart, 7) mouse brain and 8) mouse lung tissue lysate with MPI antibody at 0.5ug/ml. Predicted molecular weight ~47 kDa.
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Western blot testing of 1) rat testis and 2) human HeLa lysate with MPI antibody at 0.5ug/ml. Predicted molecular weight ~47 kDa.
IHC staining of FFPE human intestinal cancer with MPI antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human lung cancer with MPI antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat brain with MPI antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of 1) rat ovary and 2) mouse lung lysate with MPI antibody at 0.5ug/ml. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human A549 cells with MPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPI antibody.
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human HEK293, 4) rat brain, 5) rat lung, 6) rat heart, 7) mouse brain and 8) mouse lung tissue lysate with MPI antibody at 0.5ug/ml. Predicted molecular weight ~47 kDa.

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CodeSizePrice
NSJ-R32582-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the MPI antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Mannose-6 phosphate isomerase (MPI), also called Phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein corresponding to amino acids A2-K99 was used as the immunogen for the MPI antibody.
Limitation:
This antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P34949