NSJ Bioreagents

SMAD5 Antibody

Product Code:
 
NSJ-R31228
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SMAD5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of SMAD5 antibody and Lane 1: human K562; 2: (h) Jurkat; 3: rat PC-12; 4: (h) HeLa; 5: (h) SMMC-7721 cell lysate. Observed molecular weight: 52~60 kDa.
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IHC-P: SMAD5 antibody testing of human intestine cancer tissue
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IHC-P: SMAD5 antibody testing of human lung cancer tissue
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IHC-P: SMAD5 antibody testing of rat intestine tissue
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Western blot testing of SMAD5 antibody and Lane 1: human K562; 2: (h) Jurkat; 3: rat PC-12; 4: (h) HeLa; 5: (h) SMMC-7721 cell lysate. Observed molecular weight: 52~60 kDa.
IHC-P: SMAD5 antibody testing of human intestine cancer tissue
IHC-P: SMAD5 antibody testing of human lung cancer tissue
IHC-P: SMAD5 antibody testing of rat intestine tissue

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NSJ-R31228-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SMAD5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mother against decapentaplegic homolog 5, also called SMAD family member 5, is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins(BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human SMAD family member 5 (IPQIMPSISSRDVQPVAYEE) was used as the immunogen for this SMAD5 antibody (100% rat homology).
Limitation:
This SMAD5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99717