Phospho-specific antibodies for signal transduction
Phosphorylation is the addition of phosphate groups to proteins. Phosphate groups attach to a serine, threonine, or tyrosine residue via a kinase mediated reaction, and are important for activation of various signalling pathways.
Affinity Biosciences specialise in phospho-specific antibodies (phosphor-antibodies): affinity-purified polyclonal or monoclonal antibodies that are monospecific for a target protein with a specific phosphorylation. These antibodies provide critical insight into the investigation of complex signal transductions events.
Quality antibodies - Blocking peptide validation
Quality assurance at Affinity Biosciences is of utmost significance. Their quality peptide design is essential to generating specific, selective, and reproducible antibodies. Affinity Biosciences uses synthetic peptide haptens or recombinant protein to produce antibodies with expertise and proficiency from the beginning, thereby allowing strict quality control and highly specific targeting of particular protein regions and isoforms.
Blocking peptide validation is used in the validation of Affinity Biosciences phosphor-antibodies. It is vital for the production of phosphor-antibodies, because it ensures the site-specificity. This means the phosphorylated antibodies can and will only recognise the phosphorylated target protein at the corresponding site and non-phosphorylated target protein or target protein phosphorylated at other sites will not be recognised. Affinity Biotech provide corresponding blocking peptide for each and every antibody, so you can perform antibody validation using these blocking peptides yourself if you wish.