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An overview of the research applications of BriClone Hybridoma Cloning and Selection Supplement.
Documents
Cell Culture
NICB
BriClone has been manufactured onsite for over 25 years by research scientists at the publicly-funded National Institute for Cellular Biotechnology, (Dublin City University, Dublin, Ireland) and has a long history of application in research internationally.
Documents
Cell Culture
NICB
BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning. To demonstrate the stability of BriClone, various simulated conditions were assessed mimicking different temperature exposures. Post-fusion hybridoma production was assessed using BriClone under different conditions (overnight at room temperature) as a positive control, as well as one week stored at 4°C.
Documents
Cell Culture
NICB
BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning. Many commercial additives are available for improving the improving the efficiency of cell cloning at the post-fusion stages of hybridoma production. In the following experiment, BriClone was compared to two competitors as a supplement for post-fusion hybridoma production by assessing fusion efficiency.
Documents
Cell Culture
NICB
BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning. The use of feeder cells to support the outgrowth of hybridomas has many disadvantages, like batch-to-batch variation of the feeder cells and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium. BriClone has been specially developed to overcome these common problems in the post-fusion period.
Documents
Cell Culture
NICB
Protocol for Use for BriClone Hybridoma Cloning Supplement
Documents
Cell Culture
NICB
Material Safety Data Sheet for BriClone Hybridoma Cloning Supplement
Documents
Cell Culture
NICB
The QuickSwitch™ Quant Tetramer Kit can quantify the exchange with the new peptide for epitope discovery and verify that the resulting new specificity tetramer is suitable for cell staining. This kit is optimised for 10 peptide exchanges and multiple tests per resulting tetramer.
Documents
Tetramers
MBL
A reversible T cell exhaustion model for functional screening of immune checkpoint drug candidates.
Documents
MBL
MBLs p62 and phospho-p62 ELISA Kits come with a lysis buffer, are useful for drug screening and can be used with Human or Mouse cell lysate.
Documents
ELISA
MBL
Analyse antigen specific CD8+ T cell responses to SARS-CoV-2 quickly and easily with the MHC class I QuickSwitch™ kits bundled with SARS-CoV-2 peptides.
Documents
Tetramers
MBL
The QuickSwitch™ Quant Tetramer Kit for Class II MHCs quantifies the exchange of your peptides of interest for epitope discovery, vaccine design, and immune monitoring. Verify the newly created specificity tetramer for cell staining and functional analysis. This kit is optimized for 10 peptide exchanges and multiple tests per resulting tetramer.
Documents
Tetramers
MBL
List of murine models with their corresponding MHC tetramers.
Documents
Tetramers
MBL
Find popular targets for antigen specific T-cell detection.
Documents
Proteins
MBL
MBL provides MR1 Tetramers and has developed anti-PE and anti-APC antibodies that can be used in MHC tetramer staining.
Documents
Tetramers
MBL
Universal FGFR ligand as FGF-1 is a novel choice for organoid cultures.
Documents
Recombinant Proteins
MBL
To investigate the immune response to influenza viral infection, MBLI offers MHC Tetramers for detection of T cells specific to common viral influenza antigens.
Documents
Tetramers
MBL
MBL provides ELISA kits for inflammation/aging, cancer, neurodegenerative diseases and obesity, diabetes & lipid metabolism disorders.
Documents
ELISA
MBL
MHC tetramers are complexes of four MHC molecules, associated with a specific peptide and bound to a fluorochrome. Class II tetramers bind to a distinct population of CD4+ T cells.
Documents
Tetramers
MBL
Anti-Integrin avB6 ELISA Kit is a kit to provide a highly specific detection of anti-integrin avB6 autoantibody in human serum.
Documents
ELISA
MBL
MBLI offers novel solutions for screening MHC binding peptides and creating custom MHC tetramers.
Documents
Tetramers
MBL
A technology allowing quantitative measurement of CD1d-positive NKT cells would be a useful tool for immunology and clinical laboratory examinations. The development of MHC Tetramer technology has provided a breakthrough in the ability to follow T cell populations defined by their antigen specificity.
Documents
Tetramers
MBL
CrownVivo™ antibodies are functional grade isotype controls manufactured specifically for in vivo animal studies including antigen neutralization and pathway/blockade depletion assays during the development and validation of antibody biotherapeutics. These isotype controls are designed to avoid cross-reactivity with common preclinical species and are supplied in the exact format needed for in vivo, ex vivo, or in vitro studies.
Documents
Antibodies
MBL
Krishgen Biosystems offers a range of ELISA kits for monitoring biotherapies.
Documents
ELISA
Krishgen Biosystems
Krishgen Biosystems offer a range of vaccine titer assays.
Documents
ELISA
Krishgen Biosystems