In order to provide suspended human hepatocytes, freshly isolated hepatocytes from human liver tissue are rapidly suspended and preserved in Biopredic’s optimised hypothermic medium to maintain cell viability and functional activity during shipment period (up to 48hours).
Key features of human fresh hepatocytes in suspension
- Good availability of fresh cells with proprietary production process
- High quality product combined with optimized hypothermic shipping condition
- Stable and retained viability and expression level of metabolizing enzymes
- Greater flexibility to handle cells either in suspension or plated cell culture
- Characterization data available for each product batch sheet
- General patient information (age, gender, race, health conditions, serology)
Characterisation for fresh human hepatocytes in suspension
Biopredic provides the following data for each lot of hepatocyte suspension:
- Viability after isolation and after cold shipping period, yield, plateability after cold storage period, morphology before and after shipment
- Phase I drug-metabolizing enzymes with a cocktail of probe substrates: CYP1A2/ 3A4/ 2B6/ 2D6/ 2C19
- Phase II drug-metabolizing enzymes: UGT1A1/ UGT1A6/ UGT1A9/ UGT2B15/ SULT1A1/ SULT1A3/ 4/ SULT1E1
- Serological and sterility control data
Shipment of fresh human hepatocytes in suspension
Freshly isolated hepatocytes are suspended in an optimized hypothermic medium and then aliquoted into cryovials containing at least 10 million cells per vial (1ml).
The cell suspension is shipped the same day of the preparation (or the following day) at hypothermic condition (2-8°C).
The viability, plateability and functionality of preserved cell suspension are well maintained for at least 48h at 4°C.
Please click here to view the hepatocytes in suspension products.