To provide adherent monolayer cultures, freshly isolated and viable cells are plated at a desired seeding density onto collagen-coated culture supports according to the customer’s request/ordering.
After overnight attachment, the seeding medium is replaced with either a short-term culture medium or long-term culture medium (Lanford et al., 1989) to produce short-term (≤ 3 days) or long-term hepatocytes (≤ 6 days) cultures in which cells retain a differentiated phenotype for at least 1 week.
Both freshly prepared short-term and long-term cultured hepatocytes contain all major phase I and phase II enzymes as well as the whole gene regulation pathway necessary to investigate the potentiality of drugs to induce phase I and phase II enzymes.
Key features of human fresh plated hepatocytes
- Multi-format plated cells and custom preparation available
- Greater viability and confluence of cells in monolayer
- High expression levels of major hepatic drug-metabolizing enzymes, co-factors and cell membrane receptors
- Greater flexibility for applications with either short or long-term cultures
- Optimized packaging and shipping conditions with Biopredi’s patented technology – SEALOVAC
- Full characterization data available for each product batch sheet
- General patient information (age, gender, race, health conditions, serology)
Characterisation of human fresh plated hepatocytes
Biopredic International provides the following data for each lot of plated hepatocytes:
- Cell viability and cell culture period after isolation and seeding
- Cell morphology and culture confluence by microphotography
- Phase I drug-metabolizing enzymes with a cocktail of probe substrates: CYP1A2/ 3A4/ 2B6/ 2D6/ 2C19)
- Phase II drug-metabolizing enzymes: UGT1A1/ UGT1A6/ UGT1A9/ UGT2B15/ SULT1A1/ SULT1A3/ 4/ SULT1E1
- Serological and sterility control data
Shipment of human fresh plated hepatocytes
Plated hepatocytes cultured into multi-well plates are firstly filled with either short-term or long-term culture medium (with or without Phenol red) according to customer’s specification/request.
The pre-filled plate is then packaged using Biopredic’s patent-pending Sealovac™ Technology for the shipment at room temperature (18-30°C).
Cell quality and functional activity of packaged monolayer cells are well maintained for at least 48hours.
Please click here to view fresh plated hepatocyte products.