PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. PD-L1 can bind to PD-1, which mediates inhibitory signals from the APC.
ProSci Inc. offers an extensive range of antibodies and supporting tools to research PD-L1. Read more about their range below, or click a link to jump to a specific section:
- Risk-Free™ Single Domain Monoclonal PD-L1 Products
- Risk-Free™ Mouse Monoclonal PD-L1 Products
- PD-L1 Matched Antibody Pairs
- Additional PD-L1 Antibodies
- Related PD-L1 Products
- Further Information on PD-L1
Risk-Free™ Single Domain Monoclonal PD-L1 Products
Llama single domain antibodies (sdAbs) offer unique characteristics amenable to many applications. These sdAbs demonstrate low nanomolar binding affinity and can be used for the various applications. Note: PD-L1 antibody should be stored in working aliquots at -20 °C, stable for up to one year. As with all antibodies, care should be taken to avoid repeated freeze thaw cycles. PD-L1 Antibodies should not be exposed to prolonged high temperatures.
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Risk-Free™ Single Domain Monoclonal PD-L1 Set
| Product Set | Size | Contents | Description |
|---|---|---|---|
| PD-L1 Single Domain Set (PSI-SD8500) | 20ug/ clone | PSI-SD8585, PSI-SD8623, PSI-SD8639, PSI-SD8641 | PD-L1 antibodies were raised against a recombinant protein corresponding to amino acids 19 - 239 of human PD-L1. |
Risk-Free™ Single Domain Monoclonal PD-L1 Antibodies
| Cat. Number | Clone | ELISA | WB | ICC | IF | IHC | Flow | Functional |
|---|---|---|---|---|---|---|---|---|
| PSI-SD8585 | F1A12 | ++++ | - | +++ | ? | ? | ++ | - |
| PSI-SD8623 | F1C9 | ++++ | - | +++ | ? | ? | ++ | - |
| PSI-SD8639 | F6A9 | - | - | +++ | ? | ? | ++ | +++ |
| PSI-SD8641 | F2G2 | ++++ | - | ++ | ? | ? | - | - |
Click specific applications to see respective validation image.
Risk-Free™ Mouse Monoclonal PD-L1 Products
ProSci’s Risk-Free™ antibodies are monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are guaranteed to work for their approved applications.
Risk-Free™ Mouse Monoclonal PD-L1 Set
ProSci’s Risk-Free™ Sets consists of several mAbs with different binding features allowing selection of the best clone.
| Product Set | Size | Contents | Description |
|---|---|---|---|
| Risk-Free PD-L1 Set (PSI-RF16030) | 20ug/ clone | PSI-RF16031, PSI-RF16032, PSI-RF16035, PSI-RF16036, PSI-RF16037, PSI-RF16038 | PD-L1 antibodies were raised against the extracellular domain of human PD-L1. |
Risk-Free™ Mouse Monoclonal PD-L1 Antibodies
ProSci Risk-Free™ PD-L1 antibodies are rigorously tested and guaranteed to work for their designated applications.
"-"=Negative, "+"=Fair, "++"=Average, "+++"=Good, "++++"=Excellent.Click specific applications to see respective validation image.
PD-L1 Matched Antibody Pairs
A sandwich ELISA was performed using the anti-PD-L1 mAbs PSI-RF16031, PSI-RF16032, PSI-RF16033, and PSI-RF16034 as the capture antibodies for the PD-1 extracellular domain antigen with anti-PD-1 sdAbs PSI-SD8585, PSI-SD8623, PSI-SD8639, and PSI-SD8641 as the detection antibodies.
| Target | Matched Pair | Capture Antibody | Detection Antibody | Host |
|---|---|---|---|---|
| PD-L1 Human Matched Antibody Pair #1 | PSI-RFP-0301 | PSI-RF16031 | PSI-SD8585 | Mouse - Llama |
| PD-1 Human Matched Antibody Pair #2 | PSI-RFP-0302 | PSI-RF16031 | PSI-SD8623 | Mouse - Llama |
| PD-1 Human Matched Antibody Pair #3 | PSI-RFP-0303 | RF16031 | PSI-SD8639 | Mouse - Llama |
| PD-1 Human Matched Antibody Pair #4 | PSI-RFP-0304 | PSI-RFP-0302 | PSI-SD8585 | Mouse - Llama |
| PD-1 Human Matched Antibody Pair #5 | PSI-RFP-0305 | PSI-RFP-0302 | PSI-SD8623 | Mouse - Llama |
| PD-1 Human Matched Antibody Pair #6 | PSI-RFP-0306 | PSI-RFP-0302 | PSI-SD8639 | Mouse - Llama |
Additional PD-L1 Antibodies
Monoclonal antibodies are highly specific and recognize only one epitope on an antigen which generates consistent performance in quantitative detection applications regardless of production batch. Polyclonal antibodies have high affinity and recognize different epitopes on a specific antigen. Note: These antibodies can be stored at -20˚C and are stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Monoclonal PD-1 Products
| Cat. Number | Name | Applications |
|---|---|---|
| PSI-76-958 | CD274 Antibody [10F.9G2] | Flow, Func |
| PSI-76-960 | CD274 Antibody [10F.9G2] (PE) | Flow |
Polyclonal PD-1 Products
| Cat. Number | Antigen Range | ELISA | WB | ICC | IF | IHC | Flow |
|---|---|---|---|---|---|---|---|
| PSI-4059 | 60 - 110 | ++++ | +++ | ++++ | +++ | +++ | +++ |
| PSI-46-156 | 240 - 290 | ? | +++ | ? | ? | ++++ | ? |
Related PD-1 Products
Recombinant Proteins
| Cat. Number | Sequence | Fusion Tag | Species | Tissue/ Cell |
|---|---|---|---|---|
| PSI-90-429 | Aa 19 - 238 | Fc Tag | Human | CHO |
| PSI-90-430 | Aa 19 - 238 | Fc Tag | Human [Non-Lytic] | CHO |
| PSI-90-431 | Aa 19 - 237 | Fc Tag | Mouse | CHO |
| PSI-90-432 | Aa 19 - 237 | Fc Tag | Mouse [Non-Lytic] | CHO |
| PSI-91-293 | Phe 19 - Thr 239 | C-6 His tag | Human | Human |
| PSI-92-625 | Phe 19 - Thr 239 | C-Flag Tag | Human | Human |
| PSI-96-598 | Phe 19 - Arg 238 | C-Fc Tag | Human | HEK293 |
| PSI-96-599 | Phe 19 - Arg 238 | C-Twin Strep Tag | Human | HEK293 |
| PSI-96-606 | Phe 19 - Arg 238 | His Tag | Human | HEK293 |
| PSI-96-608 | Phe 19 - Thr 238 | His Tag | Human | HEK293 |
| PSI-96-610 | Phe 19 - Thr 238 | C-Fc Tag | Mouse | HEK293 |
Peptides
| Cat. Number | Location | Species |
|---|---|---|
| PSI-4059P | 17 amino acids near the center of human PD-L1 | Human |
| PSI-46-156P | PD-L1 peptide corresponds to 12 amino acids of PD-L1 | Human |
Antigen
| Cat. Number | Product Type | Species | Tissue/ Cell |
|---|---|---|---|
| PSI-RF16030-01 | Recombinant Protein | Human | HEK293 |
Lysates
| Cat. Number | Product Type | Species | Tissue/ Cell |
|---|---|---|---|
| PSI-RF10001-04 | Positive Control (Tissue Lysate) | Human | Tonsil |
| PSI-RF10001-06 | Positive Control (Cell Lysate) | Human | Raji |
| PSI-RF10001-02 | Negative Control (Cell Lysate) | Human | HEK293 |
Slides
| Cat. Number | Product Type | Species | Tissue/ Cell |
|---|---|---|---|
| PSI-RF10002-04 | Positive Control (Cells) | Human | Raji |
| PSI-RF10002-02 | Negative Control (Cells) | Human | HEK293 |
Caltag Medsystems offers a multitude of Immune Checkpoint research products from ProSci and other supplier. If you are looking for something else, please contact us and we will be happy to help.
Further Information on PD-1
- Blog: Problems with Immunotherapy
- Genecards: PD-L1 Database
- Immune Checkpoints Flyer
- Video: Cancer Immunotherapy: PD-1 & PD-l1
ProSci products are distributed in the UK by Caltag Medsystems. If you have any questions about the products in this blog, please contact us.