UK and Ireland Distributor of MBL International - Reagents for the Detection of Autoimmune Diseases

Autoimmune Indirect Fluorescent Antibody Assays (IFA)

Antinuclear antibodies (ANAs) are a group of autoantibodies characterised by specificity for numerous antigenic determinants of cell nuclei. ANAs have been proven to be very useful as disease markers, primarily for diagnostic screening and also to monitor the course of connective tissue diseases.

Why Use IFA?

The immunofluorescence ANA test is the preferred method for ANA testing. Since 2009, the American College of Rheumatology has recommended that the immunofluoresence ANA test should remain the gold standard for ANA testing*

Autoimmune Blistering Diseases are a group of disorders associated with autoantibodies that re directed against desmosomal structural proteins. MBL offers ELISA kits for detection and monitoring of these diseases including Bullous Pemphigoid and Epidermolysis Bullosa Acquisita.

The Indirect fluorescent antibody (IFA) test is sensitive, screens for a wide variety of known and unknown autoantibodies and through pattern recognition offers insights into the probable identity of the antigen and associated autoimmune disorder. The ANA test system uses Hep-2 tissue culture cells as a substrate for the determination of antinuclear antibodies in human serum and is intended for use as an aid in the diagnosis of certain autoimmune diseases.

ANA Test Systems

120 test kit60 test kit
10 x 12-Well Antigen Substrate Slides10 x 6-Well Antigen Substrate Slides
1 x 0.5 ml Positive Control Serum1 x 0.5 ml Positive Control Serum
1 x 0.5 ml Negative Control Serum1 x 0.5 ml Negative Control Serum
2 x 3.5 ml Conjugate1 x 3.5 ml Conjugate
1 x 3.5 ml Mounting Medium1 x 3.5 ml Mounting Medium
2 x 1 liter packets PBS2 x 1 liter packets PBS
Product CodeProduct Name Size
ANK-120ANA (HEp-2) Test System 120 tests
ANK-60ANA (HEp-2) Test System 60 tests

ANA Cell Culture Substrate Slides

  • Each lot is tested with a panel of titered control sera to ensure sensitivity from lot to lot
  • Each lot is tested, not only for pattern types, but also for specific Antinuclear Antibodies
  • Mitotic cells are present for detection of Centromere Antibodies
  • Sensitive for detection of low titered specific antibodies
  • Specific for detection of SS-A and other antibodies
Product CodeProduct Name Size
AN-1016Antinuclear Antibody (HEp-2) 16 wells
AN-1012Antinuclear Antibody (HEp-2) 12 wells
AN-1006Antinuclear Antibody (HEp-2) 6 wells

ANA Control Sera

Positive Control Sera are prepared to give a 2+ to 4+ fluorescence when used as supplied (undiluted) in BION's Immunofluorescent Test Systems. Negative Control Sera are prepared to give less than a 1+ fluorescence when used as supplied (undiluted) in BION's Immunofluorescent Test Systems.

Product CodeProduct Name Size
ANP-1040ANA Positive Control (Homogeneous) 0.5ml
ANN-1010ANA Negative Control 0.5ml
NUC-1042ANA Nucleolar Control 0.5ml
ACA-1043ANA Anti-Centromere Control (ACA) 0.5ml
RNP-1044ANA Ribonucleoprotein Control (RNP) 0.5ml
SSA-1045ANA Sjogren's Syndrome A Control (SS-A) 0.5ml
SSB-1047ANA Sjogren's Syndrome B Control (SS-B) 0.5ml
SCL-1046ANA Scleroderma-70 Control (Scl-70) 0.5ml

ANA Components and Conjugates

Fluorescent Antibody Conjugates

Ready to use dropper bottle of FITC labelled goat anti-human IgG (heavy and light chain specific), to detect IgG, IgA and IgM with Evans Blue counterstain.

Product CodeProduct NameSize
CCP-9970Anti-Human IgG Conjugate3.5ml
CCP-9920Anti-Human IgG Conjugate20ml
CCP-9940Anti-Human IgG Conjugate40 ml
CCP-9960Anti-Human IgG Conjugate60ml

Phosphate Buffered Saline (PBS)

Dry packets to make 1 or 5l of PBS

Product CodeProduct NameSize
PBS-9990Phosphate Buffered Saline, 1l1 packet
PBS-9991Phosphate Buffered Saline, 5l1 packet

Mounting Medium

Dropper bottle containing phosphate buffered glycerol of pH 7.4

Product CodeProduct NameSize
MM-9985Mounting Medium 3.5ml dropper bottle
MM-9986Mounting Medium 20ml dropper bottle

IFA Diluent

IFA dilute is intended to reduce the nonspecific fluorescence associated with some serum specimens.

 Product CodeProduct NameSize
DIL-9993IFA Diluent60ml
DIL-9994IFA Diluent125ml
DIL-9995IFA Diluent250ml

*American College of Rheumatology, Position Statement on the Methodology of testing for

Antinuclear Antibodies Diseases. Available at www.rheumatology.org/practice/clinical/position/ana_position_stmt.pdf

Autoimmune Blistering Disease ELISAs

Autoimmune Blistering Diseases are a group of disorders associated with autoantibodies that are directed against desmosomal structural proteins. MBL offers ELISA kits for detection and monitoring of these diseases including Bullous Pemphigoid and Epidermolysis Bullosa Acquisita

  • Highly Specific
  • Parallel fluctuation with disease activity
  • High Sensitivity
  • Economical breakaway strips
Product CodeProduct NameSize
RG-7845R2Anti TypeVII Collagen ELISA Kit48 well
RG-M7593-D*Dsg1 and Dsg3 ELISA test system48 well
RG-7880EC-DMESACUP-2 Desmoglein Test 'Dsg1' CE48 well
RG-7885EC-DMESACUP-2 Desmoglein Test 'Dsg3' CE48 well
RG-M7612-D*MESACUP BP180 ELISA Kit48 well
RG-M7613-D*MESACUPBP230 ELISA Kit48 well
RG-7115RAnti Skin Profile ELISA Kit8x12 wells

Anti Skin Profile ELISA Kit - 1 Assay for 5 Autoantibodies

  • Single assay for the detection of five anti-skin-specific  antibodies
  • Qualitative detection anti-Dsg1, anti-Dsg3,  anti-BP180, anti-BP230 and anti-TypeVII  collagen in human serum.
  • A single patient's sample can be tested for all 5 antigens on 12 occasions
  • Only commercial anti skin panel ELISA kit for diagnosis and monitoring of ABDs

The Anti-Skin Profile ELISA Kit can simultaneously detect five anti-skin-specific antibodies (anti-Dsg1, anti-Dsg3, anti-BP180, anti-BP230 and anti-Type VII collagen) in human serum. Wells of a microtitre strip (8 wells) are separately coated with 5 different recombinant antigens.

MBLI's Anti-Skin Profile ELISA Kit helps in the diagnosis of patients with pemphigus (PV/PF), bullous pemphigoid (BP) and EBA, Dsg-1, Dsg-3, BP180, BP230 and Type VII Collagen

Product CodeProduct NameSize
RG-7115RAnti Skin Profile ELISA Kit8x12 wells

Compare the Anti Skin Profile ELISA Kit - 1 Assay for 5 Autoantibodies to individual ELISAs

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