d-Desthiobiotin
| Code | Size | Price |
|---|
| CDX-D0272-M500 | 500 mg | £126.00 |
Quantity:
| CDX-D0272-G001 | 1 g | £205.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
Short Term Storage: +4?C. Long Term Storage: +4?C
Documents
Further Information
Alternate Names/Synonyms:
5-Methyl-2-oxo-4-imidazolidinehexanoic acid; Desthiobiotin
Appearance:
White to off-white powder.
CAS:
533-48-2
EClass:
32160000
Form (Short):
solid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C10H18N2O3/c1-7-8(12-10(15)11-7)5-3-2-4-6-9(13)14/h7-8H,2-6H2,1H3,(H,13,14)(H2,11,12,15)/t7-,8+/m0/s1
InChiKey:
AUTOLBMXDDTRRT-JGVFFNPUSA-N
Long Description:
Chemical. CAS: 533-48-2. Formula: C10H18N2O3. Molecular Weight: 214.26. D-Desthiobiotin is a protein cross-linking agent and a precursor in the synthesis of biotin. Desthiobiotin is a modified form of biotin that binds less tightly to avidin and streptavidin than biotin while still providing excellent specificity in affinity purification methods. Unlike biomolecules that are labeled with biotin, proteins and other targets that are labeled with desthiobiotin can be eluted without harsh, denaturing conditions. D-Desthiobiotin is used in affinity chromatography and protein chromatography, and can be used for protein and cell labeling, detection and isolation. It has been used in the preparation of agarose matrices for affinity-based isolation of streptavidin-fluorophore conjugates. Desthiobiotin is an efficient competitor for the mild elution of Strep-tag? and TwinStrep-tag? fusion proteins from Strep-Tactin? columns. For elution 2.5 mM desthiobiotin is added to the buffer.
MDL:
MFCD00213804
Molecular Formula:
C10H18N2O3
Molecular Weight:
214.26
Package Type:
Vial
Product Description:
D-Desthiobiotin is a protein cross-linking agent and a precursor in the synthesis of biotin. Desthiobiotin is a modified form of biotin that binds less tightly to avidin and streptavidin than biotin while still providing excellent specificity in affinity purification methods. Unlike biomolecules that are labeled with biotin, proteins and other targets that are labeled with desthiobiotin can be eluted without harsh, denaturing conditions. D-Desthiobiotin is used in affinity chromatography and protein chromatography, and can be used for protein and cell labeling, detection and isolation. It has been used in the preparation of agarose matrices for affinity-based isolation of streptavidin-fluorophore conjugates. Desthiobiotin is an efficient competitor for the mild elution of Strep-tag? and TwinStrep-tag? fusion proteins from Strep-Tactin? columns. For elution 2.5 mM desthiobiotin is added to the buffer.
Purity:
>98%
SMILES:
O=C(O)CCCCC[C@H]([C@H](C)N1)NC1=O
Solubility Chemicals:
Soluble in DMSO or DMF.
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 2 years after receipt when stored at +4?C.
References
(1) J.D. Hirsch, et al.; Anal. Biochem. 308, 343 (2002) | (2) R. Rodriguez-Melendez, et al.; J. Nutr. 133, 1259 (2003) | (3) S.C. Wu & S.L. Wong; Anal. Biochem. 331, 340 (2004) | (4) P. Guillaume, et al.; J. Immunol. 177, 3903 (2006) | (5) T.G.M. Schmidt, et al.; Protein Expr.Purif. 92, 54 (2013)