Beta-actin Antibody

ProSci
Product Code: PSI-3779
Product Group: Primary Antibodies
Supplier: ProSci

CodeSizePrice
PSI-3779-0.02mg0.02mg£150.00
Quantity:
PSI-3779-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of beta-Actin in A431, Daudi, HepG2, HL60, Jurkat, Human brain, Mouse lung, and Chicken liver lysate with beta-Actin antibody at 1 μg/mL.
2 / 3
Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 μg/mL.
3 / 3
Immunofluorescence of beta-Actin in Hela cells with beta-Actin antibody at 2 μg/mL.

Western blot analysis of beta-Actin in A431, Daudi, HepG2, HL60, Jurkat, Human brain, Mouse lung, and Chicken liver lysate with beta-Actin antibody at 1 μg/mL.
Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 μg/mL.
Immunofluorescence of beta-Actin in Hela cells with beta-Actin antibody at 2 μg/mL.

Further Information

Additional Names:
beta-Actin Antibody: Beta actin, b actin
Application Note:
b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
beta-Actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Background References:
  • Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
  • Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.
Buffer:
beta-Actin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
b-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human b-actin.
NCBI Gene ID #:
60
NCBI Official Name:
actin, beta
NCBI Official Symbol:
ACTB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH02409
Protein GI Number:
12803203
Purification:
beta-Actin Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
P60709
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
Beta-actin PeptidePSI-3779PProSci Summary Details