ATG14 Antibody

ProSci
Product Code: PSI-7043
Product Group: Primary Antibodies
Supplier: ProSci

CodeSizePrice
PSI-7043-0.02mg0.02mg£150.00
Quantity:
PSI-7043-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 μg/mL.

Western blot analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 μg/mL.

Further Information

Additional Names:
ATG14 Antibody: ATG14L, BARKOR, KIAA0831, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Barkor
Application Note:
ATG14 antibody can be used for detection of ATG14 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ATG14 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.
Background References:
  • Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
  • Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
  • Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
  • Itakura E, Kishi C, Inoue K, et al. Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 2008; 19:5360-72.
Buffer:
ATG14 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATG14 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG14.

The immunogen is located within amino acids 270 - 320 of ATG14.
NCBI Gene ID #:
22863
NCBI Official Name:
ATG14 autophagy related 14 homolog (S. cerevisiae)
NCBI Official Symbol:
ATG14
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
NP_055739
Protein GI Number:
50540545
Purification:
ATG14 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms
Swissprot #:
Q6ZNE5
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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