StemExpress - Distributed in the UK by Caltag Medsystems

StemExpress

StemExpress are a leading biospecimen provider of human peripheral blood, Leukopaks, Mobilised Leukopaks, cord blood, bone marrow, disease state products, and primary cells. They provide expediency from order to delivery and guarantee every sample arrives with the highest purity, viability, and quality researchers need to ensure confidence in their results.

What makes StemExpress different?

Peripheral Blood

Human peripheral blood is one of the most easily accessible sources for obtaining unprocessed tissue and primary cells. At StemExpress’s Stem Cell Collection Centres, healthy and diseased unprocessed human peripheral blood products are collected from IRB consented donors and shipped out directly or quickly processed at their FDA registered laboratory to ensure each primary cell type is of the highest quality. Each cell type is characterised by flow cytometry to guarantee you receive a pure and viable cell population for your research applications.

• Whole Peripheral Blood
• Peripheral Blood Leukopaks^®
• Peripheral Blood Primary Cells
• Peripheral Blood Plasma
• Peripheral Blood Serum

Whole Peripheral Blood

Let StemExpress find the right donor for your project! The extensive donor pool availability of StemExpress allows flexibility in specific donor criteria and the ability to meet your donor requests quickly.

• Whole Peripheral Blood–Bags – up to 500 mL
• Whole Peripheral Blood–Vacutainer^® – up to 250 mL
• Cryopreserved and fresh options available
• Range of anticoagulants available (ACD-A, EDTA or heparin)
• Possible to use custom collection tubes

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Peripheral Blood Leukopaks^®

StemExpress’ Peripheral Blood Leukopaks^® provide enriched peripheral blood mononuclear cells (PBMCs) with low granulocyte and red blood cells. This highly enriched PBMC fraction requires minimal downstream processing (i.e. Ficoll) and can be used directly for isolating high yields of immune cells, such as CD4+ T cells, CD8+ T cells, CD19+ B cells, monocytes, and other immune cells. High cell yields allow researchers greater reproducibility of experiments from a single donor (minimising donor-to-donor variation), a decrease in same cell variability, and scalability of experiments. In addition, up to 12 Leukopaks can be collected from the same donor in a given year, providing an ample amount of PBMCs for large-scale experiments and research studies.

Cell-rich Leukopaks are extracted using an FDA-approved apheresis machine. Once collected, fresh Leukopaks are sent out on the same day or frozen immediately to ensure a highly viable product.

• 1×10¹⁰TNCs/full bag
• Highly enriched PBMCs
• ≥95% viability (fresh)
• Variaty of sizes, from 1/10th bags to full bags
• HBV, HCV, HIV negative
• IRB approved

Figure 1. Representative flow analysis of a peripheral blood leukopak after RBC lysis.

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Peripheral Blood Primary Cells

Human peripheral blood contains a vast array of hematopoietic cells involved in defending the body against infection and disease. Each primary cell type has a specialised function and plays a pivotal role in human health. The study of these cells allows insight into ways of enhancing treatment and development of cures for infectious and chronic diseases and is fundamental to the advancement of cellular and regenerative medicine.

StemExpress offers a wide variety of human peripheral blood primary cells from healthy IRB consented donors who are HBV, HCV, and HIV negative. Cell attributes, such as phenotype, viability, purity, and cell count, are checked to meet rigorous standards ensuring high-quality cells before leaving the facility.

Figure 2. Representative flow data of the purity of freshly isolated peripheral blood CD14 monocytes using negative selection.

• Mononuclear Cells(PBMCs)
• Granulocytes
• Dendritic Cellsand Macrophages
• Stem and Progenitor Cells
• T Cells, B Cells, Monocytes, and NK Cells
• Other subsets available

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Peripheral Blood Plasma

Human peripheral blood plasma contains a variety of cytokines, chemokines, and growth factors that affect the proliferation and function of immune cells, which allows peripheral blood plasma to be applicable in a wide variety of therapeutic uses. The plasma is obtained by spinning down fresh whole peripheral blood previously collected in a collection bag containing an anticoagulant.

• Range of anticoagulants available (ACD-A, EDTA or heparin)
• Possible to use custom collection tubes
• 10-200mL volumes available
• Cryopreserved or fresh
• Customisable packaging options

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Peripheral Blood Serum

Human peripheral blood serum contains the same components as plasma, such as cytokines, chemokines, and growth factors, but lacks blood clotting factors. To isolate serum, peripheral blood is collected into a sterile collection bag that does not contain an anticoagulant. Fresh peripheral blood is then sent to StemExpress’ FDA-registered cell manufacturing facility where the blood is allowed to coagulate. Serum is collected into 5-250mL volumes.

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Mobilised Peripheral Blood

Human Mobilised Peripheral Blood Leukopaks are collected from healthy IRB consented donors that are injected with Granulocyte-Colony Stimulating Factor (G-CSF aka Neupogen^®), Plerixafor (aka Mozobil^®), or a combination of Plerixafor and G-CSF. Mobilisation stimulates the bone marrow to produce a large number of haematopoietic and progenitor stem cells and releases these cells into the bloodstream, allowing for enriched CD34+ haematopoietic stem cells from a single donor as compared to traditional Leukopaks. Collecting CD34+ stem cells from a single donor eliminates donor-to-donor variation, allows scalability of stem cell studies, and decreases same cell variability. Mobilised Leukopaks are ideal for stem cell studies relating to regenerative medicine, immunotherapy, and transplant therapy.

Cell-rich Mobilised Leukopaks are extracted using an FDA-approved apheresis machine. Once collected, Mobilised Leukopaks are sent out on the day of collection to ensure you receive a fresh product.

• 2×10¹⁰ TNCs/full bag
• Enriched CD34+ cells
• Highly enriched PBMCs
• ≥90% viability
• HBV, HCV, HIV negative
• IRB approved

Figure 3. Representative flow panel of fresh G-CSF Mobilised Leukopak. Enriched PBMC population with low granulocytes (20.76%), high viability (97.65%), and a high percentage of CD34+ cells (1.68%).

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Diseased Peripheral Blood

StemExpress is a premier provider of human diseased peripheral blood, plasma, serum, and peripheral blood mononuclear cells (PBMCs). Using their strategically located collection centres and partnering hospitals, they have access to donors and the delivery of fresh products to researchers in need of rare blood samples.

All collections follow a standardised collection procedure to guarantee consistency from donor to donor, eliminating variability in the collection procedure. In addition, donors may be eligible for recall every 8 weeks (pending the health of the donor), which allows for the study of disease progression within a single donor.

• Whole Diseased Peripheral Blood
• Diseased Peripheral Blood Plasma
• Diseased Peripheral Blood Serum
• Diseased Peripheral Blood Mononuclear Cells

Disease Types Available

Whole Diseased Peripheral Blood

Up to 100 mL of whole diseased peripheral blood from IRB approved donors is collected in 10 mL Vacutainer^® tubes containing the anticoagulant EDTA. Custom collection tubes and other anticoagulants are available.

Contact us to know current availability

Diseased Peripheral Blood Plasma

Diseased human peripheral blood plasma contains a variety of cytokines, chemokines, and growth factors that affect the proliferation and function of immune cells, which allows peripheral blood plasma to be applicable in a wide variety of therapeutic uses. Additionally, diseased plasma may also contain DNA and proteins released by diseased cells, allowing researchers the ability to identify biomarkers that aid in the development of cost-effective screens for the early detection and diagnosis of diseases. Fresh diseased peripheral blood is sent to StemExpress on-site cell manufacturing facility where the blood is spun down and the plasma is collected into 5mL and 10mL volumes in EDTA tubes. All samples are provided in either fresh or frozen formats.

Contact us to know current availability

Diseased Peripheral Blood Serum

Human diseased peripheral blood serum contains the same components as diseased plasma, such as cytokines, chemokines, and growth factors, but lacks blood clotting factors.

Samples are collected from fresh diseased blood of IRB approved donors and sent to StemExpress on-site cell manufacturing facility where the blood is allowed to coagulate. Diseased peripheral blood serum is provided in 5mL and 10mL volumes in either fresh or frozen formats.

Contact us to know current availability

Diseased Peripheral Blood Mononuclear Cells

StemExpress provides peripheral blood mononuclear cells (PBMCs) from diseased peripheral blood from IRB approved donors for the study of a wide range of diseases. Cell attributes, such as phenotype, viability, and cell count, are checked to meet rigorous standards ensuring high-quality PBMCs before leaving the facility. Diseased cells are available in 5x10e⁶ and 1x10e⁷ cells/vial in either fresh or frozen formats.

Contact us to know current availability

Bone Marrow

StemExpress provides whole human bone marrow and bone marrow-derived primary cells. Bone marrow is collected by a certified medical professional from a maximum of four sites on the posterior iliac crest (100 mL total). Human bone marrow samples are shipped immediately or processed into a specific primary cell population at StemExpress’s on-site FDA registered laboratory.

Bone marrow is the site of haematopoiesis or the location of blood cell formation. It is rich in haematopoietic stem cells (CD34+ and CD133+ cells), the cells that give rise to white blood cells, red blood cells, and platelets, and is a key source for mesenchymal stem cells (MSCs), also called mesenchymal stromal cells, which provide the microenvironmental support for haematopoietic stem cells and produce cells of the connective tissue such as bone, cartilage, fat, and muscle.

• Whole Bone Marrow
• Bone Marrow Primary Cells

Whole Bone Marrow

Fresh human whole bone marrow is collected by a licensed medical professional from healthy IRB consented donors at one of StemExpress Collection Centres. The fluid portion of the bone marrow is aspirated from up to a maximum of 4 sites on the posterior iliac crest and collected into 10 to 100mL containers that include anticoagulants Na-Heparin or ACD-A. We also offer other anticoagulants by request.

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Bone Marrow Primary Cells

Fresh whole bone marrow previously collected is transferred to StemExpress on-site cell manufacturing facility where specific bone marrow primary cells are processed, analysed, and vialed. Cell attributes, such as phenotype, viability, purity, and cell count, are checked to meet rigorous standards, ensuring high-quality cells before leaving the facility.

Figure 4. High Quality Cells = Viability and Purity

• Mononuclear Cells
• Stem and Progenitor Cells
• Mesenchymal Stem Cells
• Myeloid Cells
• Other subsets available

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Cord Blood

StemExpress is a leading supplier of human cord blood and primary cells. Human cord blood consists of immune cells and is considered a rich source of hematopoietic stem and progenitor cells. Collection of cord blood is a noninvasive and painless procedure performed that consists on needle aspirating the blood from the umbilical cord vein into a sterile collection bag containing the anticoagulant citrate phosphate double dextrose (CP2D). Cord blood is shipped directly after collection or processed into a specific primary cell population at GLP regulatory-compliant laboratory.

• Whole Cord Blood
• Cord Blood Primary Cells
• Cord Blood Plasma

Whole Cord Blood

Fresh whole cord blood, that is HIV, HBV, and HCV negative is collected in sterile collection bags containing the anticoagulant citrate phosphate double dextrose (CPD). Multiple sizes based on TNC count available.

• Gender and ethnicity information available
• HLA, RhD, and ABO typing available
• Infectious disease screening of cord blood (HIV, HBV, and HCV)
• Additional infectious disease screening (CMV, etc.)

Contact us to know current availability

Cord Blood Primary Cells

Fresh whole cord blood is transferred to StemExpress’s cell manufacturing facility where specific primary cells are processed, analysed, and vialed. Cell attributes, such as phenotype, viability, purity, and cell count, are checked to meet rigorous standards ensuring high-quality cells before leaving the facility. FOR RESEARCH USE ONLY – NOT FOR HUMAN USE

Figure 5. Representative flow data fo the vialability and purity of enriched CD34+ cells from cord blood mononuclear cells prior to cryopreservation.

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Cord Blood Plasma

Human cord blood plasma contains a variety of cytokines, chemokines, and growth factors that affect the proliferation and function of immune cells, allowing cord blood plasma to be applicable in a wide variety of therapeutic uses. Fresh whole cord blood is spun down and the plasma is collected in 10-50mL volumes.

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