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Instruction video of the HITAlert™ Kit from IQ Products
Videos
Specialities
IQ Products
Instruction video of the FMH QuikQuant™ from IQ Products
Videos
Perinatal
IQ Products
Instruction video of the Fetal Cell Count™ kit from IQ Products
Videos
Perinatal
IQ Products
Collagen hybridizing peptides allow for visualization of fibrotic tissue or any tissues with an increase of collagen turnover.
Videos
Peptides
3Helix
Collagen is the major building block in all load bearing tissues including tendon, ligament, bone, and cartilage. Mechanical injury to these tissues causes changes in collagen structure which can initiate pathological processes without detectable morphological changes at the macroscale. Using CHPs you can now visualize the mechanical damage to the triple helix.
Videos
Peptides
3Helix
The skeletal system has a large quantity of collagen, making visualization of skeletal development, disease and turnover with CHPs a perfect use case. Find out more at 3helix.com
Videos
Peptides
3Helix
Collagen has a unique triple helical structure that is unfolded in tissues during diseases, development, mechanical injury, and decellularization. 3Helix commercializes labeled collagen hybridizing peptide (CHP) that specifically binds to such unfolded collagen chains.
Videos
Peptides
3Helix
Every decellularization method may alter the composition and the micro-architecture of ECM, causing variations of the biological activity and mechanical integrity of the decellularized material. CHP specifically detects denatured collagen in the decellularized tissue/organ, enabling assessment of the effect of decellularization upon ECM at the molecular level.
Videos
Peptides
3Helix
Despite overwhelming evidence demonstrating the important role of the extracellular matrix in regulating cell behavior, traditional cell culture strategies do little to recreate a natural microenvironment. Presently, standard culture conditions inevitably lead to phenotypic drift, spontaneous differentiation, replicative senescence, and aberrant phenotypes that are unlike those found in the body. This may lead to experimental artifact in basic research or suboptimal clinical outcomes in translational research. To address this challenge, we focused our efforts on developing in vitro culture substrates that closely mimic natural microenvironments. Here we describe the development of scale-able, tissue-specific, cell-derived extracellular matrices for cell culture, and their applications in basic and translation research.
Videos
Cell Culture
StemBioSys
A tutorial on how to properly thaw and plate cryopreserved cells from ZenBio
Videos
Cell Culture
ZenBio
The benefits of the unique CELLvo Matrix, the only natural cell culture substrate, will be discussed. The webinar describes the current challenges in removing cells from their native tissue and culturing them in a completely foreign environment such as tissue culture plastic. CELLvo Matrix addresses this by providing a 'home' that mimics the native cell derived micro-environment that they came from. It promotes cell adhesion with better isolation and proliferation of primary cells and enhanced cellular responsiveness to experimental variables.
Videos
Cell Culture
StemBioSys
For over a decade, ScienCell Research Laboratories has helped researchers with their cell culture experiments by providing expert advice. Our webinar will discuss primary cell culture optimization techniques and why primary cells are necessary for validation of cell line studies and in vivo experiments. In addition, we will discuss how you can advance your primary cell research by using our gene expression profiling kits.
Videos
Cell Culture
Sciencell
qPCR can be a powerful tool for analyzing gene expression. When designed properly, however its strength relies on the quality of the primers used in it. Our scientists know this and follow stringent guidelines to design primers for our GeneQuery qPCR arrays. When researchers design their own primers without comprehensive knowledge of good primer design, qPCR results can be inadvertently influenced yielding unreliable and inconsistent data. Our webinar will cover guidelines used by our scientists in designing our quality GeneQuery qPCR primers arrays while highlighting our human endothelial cell biology array. An extended FAQ session for your questions and concerns will follow. Objectives: Delineate frequently overlooked primer optimization parameters during qPCR design. Demonstrate the benefits of properly optimized primers in qPCR analysis. Exemplify the ease and use of GeneQuery qPCR arrays in gene expression analysis.
Videos
Cell Culture
Sciencell
Information on the storage and use of ELISA Kits, provided by Cloud-Clone Corp. - distributed in the UK by Caltag Medsystems.
Videos
Product How To...
Cloud-Clone Corp.
In competitive ELISA, unlabeled antibody is incubated in the presence of its antigen. Then these bound antibody/antigen complexes are then added to an antigen coated well. After washing, unbound antibodies are removed. The more analytes in the sample, the less antibodies will be able to bind to antigens in the well. The signal is then detected using labeled secondary antibodies and the decrease in signal is compared to a control. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.
Videos
Methods
Abnova Corporation
Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope.
Videos
Methods
Abnova Corporation
Cell culture is the process by which cells are grown under controlled conditions. This video show you how to culture attached cells from cell thrawing, maintain, to cell freezing.
Videos
Methods
Abnova Corporation
Polymerase Chain Reaction (PCR) is a technique to amplify few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps (Denaturation, Annealing and Extension) in a PCR and repeat for 30 or 40 cycles
Videos
Methods
Abnova Corporation
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies.
Videos
Methods
Abnova Corporation
Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues. It is widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.
Videos
Methods
Abnova Corporation
Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is reverse transcribed to its DNA complement (complementary DNA, or cDNA) using reverse transcriptase and primers. The second step is amplification using traditional or real-time PCR.
Videos
Methods
Abnova Corporation
Real-time PCR, also called quantitative real time PCR (Q-PCR/qPCR), is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample.
Videos
Methods
Abnova Corporation
MBL International Corporation offers a line of highly specific antibodies conjugated to magnetic beads. This method is an advantage over conventional immunoprecipitation of tagged proteins because it eliminates time consuming centrifugation steps. The magnetic beads are covalently bound to a Tag antibody and the beads are easily isolated using a magnetic rack.
Videos
Immunology
MBL
Educational video for the RIP-Chip assay technology for microRNA. Identify miRNAs bound to their mRNA targets.
Videos
Methods
MBL
MBL has incorporated the patented mutation of the a3 domain in their HLA class I Tetramers and is the only manufacturer of MHC multimers with this technology to enhance specificity for the antigen-specific T cell receptor.
Videos
Immunology
MBL