WFDC2 Antibody / HE4

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NSJ Bioreagents
Product Code: NSJ-RQ4465
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4465-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HE4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) human HeLa, 2) human MDA-MB-231, 3) human MDA-MB-451, 4) rat thymus, 5) mouse testis and 6) mouse thymus lysate with HE4 antibody at 0.5ug/ml. Predicted molecular weight: 13-25 depending on glycosylation level.
2 / 4
IHC testing of FFPE rat small intestine tissue with HE4 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 4
IHC testing of FFPE rat spleen tissue with HE4 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 4
IHC testing of FFPE mouse spleen tissue with HE4 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of 1) human HeLa, 2) human MDA-MB-231, 3) human MDA-MB-451, 4) rat thymus, 5) mouse testis and 6) mouse thymus lysate with HE4 antibody at 0.5ug/ml. Predicted molecular weight: 13-25 depending on glycosylation level.
IHC testing of FFPE rat small intestine tissue with HE4 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat spleen tissue with HE4 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE mouse spleen tissue with HE4 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the HE4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human recombinant protein (amino acids E31-F124) was used as the immunogen for the HE4 antibody.
Limitation:
This HE4 antibody is available for research use only.
Localization:
Secreted, cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q14508