HSP105 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4470
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4470-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the HSP105 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) placenta, 3) COLO-320, 4) SGC-7901, 5) HepG2, 6) K562, 7) Jurkat and 8) SK-OV-3 lysate with HSP105 antibody at 0.5ug/ml. Expected molecular weight: 105-110 kDa.
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Western blot testing of 1) rat brain, 2) rat lung, 3) mouse brain, 4) mouse lung and 5) mouse NIH3T3 lysate with HSP105 antibody at 0.5ug/ml. Expected molecular weight: 105-110 kDa.
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IHC staining of FFPE human intestinal cancer with HSP105 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human 1) HeLa, 2) placenta, 3) COLO-320, 4) SGC-7901, 5) HepG2, 6) K562, 7) Jurkat and 8) SK-OV-3 lysate with HSP105 antibody at 0.5ug/ml. Expected molecular weight: 105-110 kDa.
Western blot testing of 1) rat brain, 2) rat lung, 3) mouse brain, 4) mouse lung and 5) mouse NIH3T3 lysate with HSP105 antibody at 0.5ug/ml. Expected molecular weight: 105-110 kDa.
IHC staining of FFPE human intestinal cancer with HSP105 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-3ug/ml,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the HSP105 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human Heat Shock Protein 105 recombinant protein (amino acids Y653-D858) was used as the immunogen for the HSP105 antibody.
Limitation:
This HSP105 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q92598