Smoothelin Antibody

NSJ Bioreagents
Product Code: NSJ-RQ4473
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4473-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
After reconstitution, the Smoothelin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) COLO-320, 3) SW620, 4) K562, 5) A375 and 6) mouse smooth muscle (intestine) lysate with Smoothelin antibody at 0.5ug/ml. Predicted molecular weight ~99 kDa but commonly observed at ~110 kDa (long form).

Western blot testing of human 1) HeLa, 2) COLO-320, 3) SW620, 4) K562, 5) A375 and 6) mouse smooth muscle (intestine) lysate with Smoothelin antibody at 0.5ug/ml. Predicted molecular weight ~99 kDa but commonly observed at ~110 kDa (long form).

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the Smoothelin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Smoothelin is a protein that in humans is encoded by the SMTN gene. This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QELEREEEALASKRFRAERQDNKENWLHSQQREAEQR were used as the immunogen for the Smoothelin antibody. This sequence is specific to the long form but not found in the short form of the protein.
Limitation:
This Smoothelin antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P53814