Calcium Sensing Receptor Antibody / CASR

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NSJ Bioreagents
Product Code: NSJ-RQ4484
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4484-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 11E9-
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the CASR antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) A549, 3) 22RV1, 4) HepG2 and 5) Caco-2 lysate with CASR antibody at 0.5ug/ml. Predicted molecular weight ~121 kDa, but is routinely observed at up to ~160 kDa.
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Flow cytometry testing of mouse Neuro-2a cells with CASR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CASR antibody.
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Western blot testing of human 1) HeLa, 2) A549, 3) 22RV1, 4) HepG2 and 5) Caco-2 lysate with CASR antibody at 0.5ug/ml. Predicted molecular weight ~121 kDa, but is routinely observed at up to ~160 kDa.
Flow cytometry testing of mouse Neuro-2a cells with CASR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CASR antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml (human),Flow Cytometry: 1-3ug/1xmillion cells (mouse)
Application Note:
Optimal dilution of the CASR antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The calcium-sensing receptor (CaSR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human CASR recombinant protein (amino acids Q926-S1078) was used as the immunogen for the CASR antibody.
Limitation:
This CASR antibody is available for research use only.
Localization:
Cell membrane
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P41180