RPA70 Antibody / RPA1

Western blot: 1:500-1:1000

Optimal dilution of the RPA70 antibody should be determined by the researcher.

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. [UniProt]

Purified

Antibody in PBS with 0.02% sodium azide and 50% glycerol

A synthetic peptide from human RPA70 was used as the immunogen for the RPA70 antibody.

This RPA70 antibody is available for research use only.

Protein A affinity

Human

P27694