ARSA Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4495
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4495-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4C10
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ARSA antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human 1) A375, 2) A549 and 3) SMMC-7721 lysate with ARSA antibdoy at 0.5ug/ml. Expected molecular weight: 53-63 kDa.
2 / 5
Western blot testing of 1) rat testis, 2) rat liver, 3) rat brain, 4) rat lung, 5) mouse testis, 6) mouse liver, 7) mouse brain, 8) mouse lung and 9) mouse HEPA1-6 lysate with ARSA antibody at 0.5ug/ml. Expected molecular weight: 53-63 kDa.
3 / 5
Flow cytometry testing of mouse ANA-1 cells with ARSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARSA antibody.
4 / 5
Flow cytometry testing of human Raji cells with ARSA antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARSA antibody.
5 / 5
IHC staining of FFPE human lung cancer with ARSA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of human 1) A375, 2) A549 and 3) SMMC-7721 lysate with ARSA antibdoy at 0.5ug/ml. Expected molecular weight: 53-63 kDa.
Western blot testing of 1) rat testis, 2) rat liver, 3) rat brain, 4) rat lung, 5) mouse testis, 6) mouse liver, 7) mouse brain, 8) mouse lung and 9) mouse HEPA1-6 lysate with ARSA antibody at 0.5ug/ml. Expected molecular weight: 53-63 kDa.
Flow cytometry testing of mouse ANA-1 cells with ARSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARSA antibody.
Flow cytometry testing of human Raji cells with ARSA antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARSA antibody.
IHC staining of FFPE human lung cancer with ARSA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow Cytometry: 1-3ug/million cells,Immunohistochemistry: 1-2ug/ml
Application Note:
Optimal dilution of the ARSA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. ARSA is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QALKQLQLLKAQLDAAVTFGPSQVARGED were used as the immunogen for the ARSA antibody.
Limitation:
This ARSA antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P15289