Cytochrome C Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4499
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4499-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 5F10
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cytochrome C antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human K562 cells with Cytochrome C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytochrome C antibody.
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Flow cytometry testing of human A431 cells with Cytochrome C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytochrome C antibody.
3 / 8
Western blot testing of human 1) HeLa, 2) HepG2, 3) K562 and 4) Caco-2 lysate with Cytochrome C antibody at 0.5ug/ml. Expected molecular weight: 12-14 kDa.
4 / 8
Western blot testing of 1) rat brain, 2) rat heart, 3) rat kidney, 4) rat testis, 5) mouse brain, 6) mouse heart, 7) mouse kidney, 8) mouse testis and 9) mouse Neuro-2a lysate with Cytochrome C antibody at 0.5ug/ml. Expected molecular weight: 12-14 kDa.
5 / 8
IHC testing of FFPE human breast cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human intestinal cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human lung cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Immunofluorescent staining of FFPE human MCF7 cells with Cytochrome C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human K562 cells with Cytochrome C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytochrome C antibody.
Flow cytometry testing of human A431 cells with Cytochrome C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytochrome C antibody.
Western blot testing of human 1) HeLa, 2) HepG2, 3) K562 and 4) Caco-2 lysate with Cytochrome C antibody at 0.5ug/ml. Expected molecular weight: 12-14 kDa.
Western blot testing of 1) rat brain, 2) rat heart, 3) rat kidney, 4) rat testis, 5) mouse brain, 6) mouse heart, 7) mouse kidney, 8) mouse testis and 9) mouse Neuro-2a lysate with Cytochrome C antibody at 0.5ug/ml. Expected molecular weight: 12-14 kDa.
IHC testing of FFPE human breast cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human intestinal cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human lung cancer with Cytochrome C antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of FFPE human MCF7 cells with Cytochrome C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow Cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the Cytochrome C antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human Cytochrome C recombinant protein (amino acids G2-E105) was used as the immunogen for the Cytochrome C antibody.
Limitation:
This Cytochrome C antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P99999