Histone H2AX Antibody (pS139)

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4503
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4503-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: AbH41
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the phospho-Histone H2AX antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of human HeLa cells treated and untreated with H2O2 using phospho-Histone H2AX antibody (green) and DAPI nuclear stain (blue).
2 / 3
Western blot testing of etoposide-treated human Jurkat lysate with phospho-Histone H2AX antibody.
3 / 3
IHC testing of FFPE mouse liver with phospho-Histone H2AX antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
❮❯

Immunofluorescent staining of human HeLa cells treated and untreated with H2O2 using phospho-Histone H2AX antibody (green) and DAPI nuclear stain (blue).
Western blot testing of etoposide-treated human Jurkat lysate with phospho-Histone H2AX antibody.
IHC testing of FFPE mouse liver with phospho-Histone H2AX antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000ug/ml,IHC (FFPE): 1:50-1:200,IF/ICC: 1:50-1:200
Application Note:
Optimal dilution of the phospho-Histone H2AX antibody should be determined by the researcher.
Description:
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
The amino acids surrounding a phosphorylated serine at position 139 were used as the immunogen for the phospho-Histone H2AX antibody.
Limitation:
This phospho-Histone H2AX antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P16104