APE1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4515
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4515-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 5C11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the APE1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE human intestinal cancer with APE1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 2
Western blot testing of human 1) HeLa, 2) MCF7, 3) COLO-320, 4) HepG2, 5) rabbit IgG, 6) marker, 7) A549, 8) PANC-1, 9) 22RV1 and 10) MDA-MB-453 lysate with APE1 antibody at 1ug/ml. Predicted molecular weight ~38 kDa.
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IHC testing of FFPE human intestinal cancer with APE1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) HeLa, 2) MCF7, 3) COLO-320, 4) HepG2, 5) rabbit IgG, 6) marker, 7) A549, 8) PANC-1, 9) 22RV1 and 10) MDA-MB-453 lysate with APE1 antibody at 1ug/ml. Predicted molecular weight ~38 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the APE1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human APE1 recombinant protein (amino acids P2-L318) was used as the immunogen for the APE1 antibody.
Limitation:
This APE1 antibody is available for research use only.
Localization:
Nucleus, cytoplasm, ER, mitochondria
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P27695