XPO1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4517
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4517-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 5G3
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the XPO1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) rabbit IgG, 6) molecular weight marker, 7) human HepG2, 8) human SMMC-7721, 9) human HeLa and 10) human Jurkat lysate with XPO1 antibody at 0.5ug/ml. Expected molecular weight ~123 kDa.
2 / 5
IHC testing of FFPE human lung cancer with XPO1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 5
IHC testing of FFPE human intestinal cancer with XPO1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 5
Flow cytometry testing of human SiHa cells with XPO1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= XPO1 antibody.
5 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with XPO1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) rabbit IgG, 6) molecular weight marker, 7) human HepG2, 8) human SMMC-7721, 9) human HeLa and 10) human Jurkat lysate with XPO1 antibody at 0.5ug/ml. Expected molecular weight ~123 kDa.
IHC testing of FFPE human lung cancer with XPO1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human intestinal cancer with XPO1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human SiHa cells with XPO1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= XPO1 antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with XPO1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/10^6 cells,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the XPO1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES)-dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human Exportin-1 recombinant protein (amino acids N966-D1071) was used as the immunogen for the XPO1 antibody.
Limitation:
This XPO1 antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O14980