Collagen IV Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4519
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4519-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3G3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Collagen IV antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE human lung cancer with Collagen IV antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
IHC testing of FFPE human placenta with Collagen IV antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
Western blot testing of human 1) HEK293T, 2) HeLa and 3) A549 cell lysate with Collagen IV antibody at 0.5ug/ml. Predicted molecular weight ~160 kDa but may be observed at a larger size due to glycosylation.
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IHC testing of FFPE human lung cancer with Collagen IV antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human placenta with Collagen IV antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) HEK293T, 2) HeLa and 3) A549 cell lysate with Collagen IV antibody at 0.5ug/ml. Predicted molecular weight ~160 kDa but may be observed at a larger size due to glycosylation.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Collagen IV antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
COL4A1, also known as ICH or Collagen alpha-1(IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human Collagen IV recombinant protein (amino acids G1445-T1669) was used as the immunogen for the Collagen IV antibody.
Limitation:
This Collagen IV antibody is available for research use only.
Localization:
Secreted
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P02462