Stefin B Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4523
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4523-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 2B6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Stefin B antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
IHC testing of FFPE human tonsil tissue with Stefin B antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
Western blot testing of human 1) U-87 MG, 2) SW620, 3) U-937 and 4) Caco-2 cell lysate with Stefin B antibody at 0.5ug/ml. Predicted molecular weight ~11 kDa.
3 / 3
Flow cytometry testing of human PC3 cells with Stefin B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Stefin B antibody.
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IHC testing of FFPE human tonsil tissue with Stefin B antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) U-87 MG, 2) SW620, 3) U-937 and 4) Caco-2 cell lysate with Stefin B antibody at 0.5ug/ml. Predicted molecular weight ~11 kDa.
Flow cytometry testing of human PC3 cells with Stefin B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Stefin B antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Stefin B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human Stefin B recombinant protein (amino acids M1-F98) was used as the immunogen for the Stefin B antibody.
Limitation:
This Stefin B antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P04080