FLAG Tag Antibody

NSJ Bioreagents
Product Code: NSJ-RQ4526
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4526-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 1E6-
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the FLAG Tag antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot test of FLAG-tagged protein with different concentrations of FLAG tag antibody.

Western blot test of FLAG-tagged protein with different concentrations of FLAG tag antibody.

Further Information

Application Details :
Western blot: 0.1-1ug/ml
Application Note:
Optimal dilution of the FLAG Tag antibody should be determined by the researcher.
Description:
FLAG-Tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It represents an early example of a nanotechnology device, in that it can be attached to a protein like a molecular handle, then used to manipulate the protein, and subsequently detached. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.
Format :
Purified
Formulation :
0.5mg/ml in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
Amino acids DYKDDDDK were used as the immunogen for the FLAG Tag antibody.
Limitation:
This FLAG Tag antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
DYKDDDDK