AES Antibody / Amino-terminal enhancer of split

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NSJ Bioreagents
Product Code: NSJ-RQ4533
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4533-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the AES antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with AES antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human HeLa cells with AES antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AES antibody.
3 / 3
Western blot testing of human U-87 MG lysate with AES antibody at 0.5ug/ml. Predicted molecular weight: ~22 kDa (AES-1), ~29 kDa (AES-2).
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Immunofluorescent staining of FFPE human U-2 OS cells with AES antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HeLa cells with AES antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AES antibody.
Western blot testing of human U-87 MG lysate with AES antibody at 0.5ug/ml. Predicted molecular weight: ~22 kDa (AES-1), ~29 kDa (AES-2).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the AES antibody should be determined by the researcher.
Description:
The AES gene is mapped to 19p13.3. The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Y69-L138 from the human protein were used as the immunogen for the AES antibody.
Limitation:
This AES antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q08117