Alpha 1 microglobulin Antibody / AMBP

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NSJ Bioreagents
Product Code: NSJ-RQ4535
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4535-100ug100ug£535.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Alpha 1 microglobulin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) placenta, 2) HEp-2, 3) HeLa, 4) A549 and 5) PANC-1 lysate with Alpha 1 microglobulin antibody at 0.5ug/ml. Predicted molecular weight: 26-28 kDa (Alpha 1 microglobulin), ~39 kDa (uncleaved AMBP). These proteins may be observed at higher molecular weights due to glycosylation.
2 / 3
IHC staining of FFPE human liver cancer with Alpha 1 microglobulin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
IHC staining of FFPE human liver cancer with Alpha 1 microglobulin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of human 1) placenta, 2) HEp-2, 3) HeLa, 4) A549 and 5) PANC-1 lysate with Alpha 1 microglobulin antibody at 0.5ug/ml. Predicted molecular weight: 26-28 kDa (Alpha 1 microglobulin), ~39 kDa (uncleaved AMBP). These proteins may be observed at higher molecular weights due to glycosylation.
IHC staining of FFPE human liver cancer with Alpha 1 microglobulin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human liver cancer with Alpha 1 microglobulin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the Alpha 1 microglobulin antibody should be determined by the researcher.
Description:
Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids G20-V203 from the human protein were used as the immunogen for the Alpha 1 microglobulin antibody.
Limitation:
This Alpha 1 microglobulin antibody is available for research use only.
Localization:
Secreted
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P02760