Calbindin Antibody / CALB1

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NSJ Bioreagents
Product Code: NSJ-RQ4541
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4541-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Calbindin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
IHC staining of FFPE mouse brain with Calbindin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 7
IHC staining of FFPE mouse kidney with Calbindin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 7
IHC staining of FFPE rat kidney with Calbindin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 7
Western blot testing of 1) rat brain, 2) rat kidney, 3) mouse brain and 4) mouse kidney lysate with Calbindin antibody at 0.5ug/ml. Predicted molecular weight: ~30 kDa, routinely observed at 27-28 kDa.
5 / 7
Flow cytometry testing of human A431 cells with Calbindin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Calbindin antibody.
6 / 7
Immunofluorescent staining of FFPE rat cerebellum tissue with Calbindin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
7 / 7
Immunofluorescent staining of FFPE rat cerebellum tissue with Calbindin antibody. HIER: steam section in pH8 EDTA for 20 min.
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IHC staining of FFPE mouse brain with Calbindin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse kidney with Calbindin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat kidney with Calbindin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of 1) rat brain, 2) rat kidney, 3) mouse brain and 4) mouse kidney lysate with Calbindin antibody at 0.5ug/ml. Predicted molecular weight: ~30 kDa, routinely observed at 27-28 kDa.
Flow cytometry testing of human A431 cells with Calbindin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Calbindin antibody.
Immunofluorescent staining of FFPE rat cerebellum tissue with Calbindin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE rat cerebellum tissue with Calbindin antibody. HIER: steam section in pH8 EDTA for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein)
Application Note:
Optimal dilution of the Calbindin antibody should be determined by the researcher.
Description:
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. And it mapped to 8q21.3. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids A2-N261 from the human protein were used as the immunogen for the Calbindin antibody.
Limitation:
This Calbindin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05937