GM130 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4570
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4570-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GM130 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 10
Western blot testing of human 1) placenta, 2) A549, 3) K562, 4) HL-60, 5) MCF-7 and 6) Caco-2 cell lysate with GM130 antibody at 0.5ug/ml. Predicted molecular weight ~130 kDa.
2 / 10
IHC staining of FFPE human esophagus squama cancer with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 10
IHC staining of FFPE human placenta with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 10
IHC staining of FFPE human tonsil with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 10
IHC staining of FFPE mouse intestine with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 10
IHC staining of FFPE mouse spleen with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
7 / 10
IHC staining of FFPE mouse brain with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
8 / 10
IHC staining of FFPE rat brain with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
9 / 10
Flow cytometry testing of human PC-3 cells with GM130 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GM130 antibody.
10 / 10
Flow cytometry testing of human U-2 OS cells with GM130 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GM130 antibody.
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Western blot testing of human 1) placenta, 2) A549, 3) K562, 4) HL-60, 5) MCF-7 and 6) Caco-2 cell lysate with GM130 antibody at 0.5ug/ml. Predicted molecular weight ~130 kDa.
IHC staining of FFPE human esophagus squama cancer with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human placenta with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human tonsil with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse intestine with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse spleen with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse brain with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat brain with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human PC-3 cells with GM130 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GM130 antibody.
Flow cytometry testing of human U-2 OS cells with GM130 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GM130 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein)
Application Note:
Optimal dilution of the GM130 antibody should be determined by the researcher.
Description:
Golgin subfamily A member 2 is a protein that in humans is encoded by the GOLGA2 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E796-E913 from the human protein were used as the immunogen for the GM130 antibody.
Limitation:
This GM130 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q08379