GOLPH3 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4571
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4571-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GOLPH3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) HeLa, 2) placenta, 3) U-2 OS, 4) Caco-2, 5) SW620 and 6) A549 lysate with GOLPH3 antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
2 / 6
Western blot testing of rat 1) heart, 2) brain, 3) stomach, 4) testis and mouse 5) heart, 6) brain, 7) stomach, 8) testis and 9) NIH3T3 lysate with GOLPH3 antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
3 / 6
IHC staining of FFPE human lung cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 6
IHC staining of FFPE human intestinal cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
IHC staining of FFPE rat intestine with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 6
IHC staining of FFPE rat brain with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of human 1) HeLa, 2) placenta, 3) U-2 OS, 4) Caco-2, 5) SW620 and 6) A549 lysate with GOLPH3 antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
Western blot testing of rat 1) heart, 2) brain, 3) stomach, 4) testis and mouse 5) heart, 6) brain, 7) stomach, 8) testis and 9) NIH3T3 lysate with GOLPH3 antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
IHC staining of FFPE human lung cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human intestinal cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat intestine with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat brain with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein)
Application Note:
Optimal dilution of the GOLPH3 antibody should be determined by the researcher.
Description:
Golgi phosphoprotein 3 (GOLPH3) is a human protein encoded by GOLPH3 gene. It is mapped to 5p13.3. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K56-K298 from the human protein were used as the immunogen for the GOLPH3 antibody.
Limitation:
This GOLPH3 antibody is available for research use only.
Localization:
Cytoplasm, plasma membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H4A6