Hsp27 Antibody / Hspb1

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NSJ Bioreagents
Product Code: NSJ-RQ4574
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4574-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Hsp27 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) rat skeletal muscle, 2) rat lung and mouse 3) spleen, 4) skeletal muscle, 5) lung, 6) liver and 7) testis lysate with Hsp27 antibody at 0.5ug/ml. Predicted molecular weight: 23-27 kDa.
2 / 4
IHC staining of FFPE mouse small intestine with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 4
IHC staining of FFPE rat small intestine with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 4
IHC staining of FFPE rat lung with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of 1) rat skeletal muscle, 2) rat lung and mouse 3) spleen, 4) skeletal muscle, 5) lung, 6) liver and 7) testis lysate with Hsp27 antibody at 0.5ug/ml. Predicted molecular weight: 23-27 kDa.
IHC staining of FFPE mouse small intestine with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat small intestine with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat lung with Hsp27 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-3ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant mouse protein)
Application Note:
Optimal dilution of the Hsp27 antibody should be determined by the researcher.
Description:
HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. It is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids M1-K209 from the mouse protein were used as the immunogen for the Hsp27 antibody.
Localization:
Predominantly cytoplasmic with some nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P14602