SHIP2 Antibody / INPPL1

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NSJ Bioreagents
Product Code: NSJ-RQ4578
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4578-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SHIP2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 10
Western blot testing of human 1) placenta, 2) HeLa, 3) U-2 OS, 4) PC-3, 5) Caco-2, 6) A549 and 7) K562 lysate with SHIP2 antibody at 0.5ug/ml. Predicted molecular weight ~139 kDa (isoform 1), ~113 kDa (isoform 2).
2 / 10
Western blot testing of rat 1) lung, 2) ovary, 3) brain and mouse 4) lung, 5) ovary and 6) brain lysate with SHIP2 antibody at 0.5ug/ml. Predicted molecular weight ~139 kDa (isoform 1), ~113 kDa (isoform 2).
3 / 10
IHC staining of FFPE human ovarian cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 10
IHC staining of FFPE human stomach cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 10
IHC staining of FFPE human placenta with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 10
IHC staining of FFPE human lung cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
7 / 10
IHC staining of FFPE mouse spleen with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
8 / 10
IHC staining of FFPE rat lung with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
9 / 10
Flow cytometry testing of human A431 cells with SHIP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SHIP2 antibody.
10 / 10
IF/ICC staining of FFPE human U-2 OS cells with SHIP2 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human 1) placenta, 2) HeLa, 3) U-2 OS, 4) PC-3, 5) Caco-2, 6) A549 and 7) K562 lysate with SHIP2 antibody at 0.5ug/ml. Predicted molecular weight ~139 kDa (isoform 1), ~113 kDa (isoform 2).
Western blot testing of rat 1) lung, 2) ovary, 3) brain and mouse 4) lung, 5) ovary and 6) brain lysate with SHIP2 antibody at 0.5ug/ml. Predicted molecular weight ~139 kDa (isoform 1), ~113 kDa (isoform 2).
IHC staining of FFPE human ovarian cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human stomach cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human placenta with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human lung cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse spleen with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat lung with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human A431 cells with SHIP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SHIP2 antibody.
IF/ICC staining of FFPE human U-2 OS cells with SHIP2 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the SHIP2 antibody should be determined by the researcher.
Description:
SH2-domain containing Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2 is an enzyme that in humans is encoded by the INPPL1 gene. The protein encoded by this gene is an SH2-containing 5'-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodelling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids R1172-K1258 from the human protein were used as the immunogen for the SHIP2 antibody.
Limitation:
This SHIP2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O15357