Lumican Antibody / LUM

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NSJ Bioreagents
Product Code: NSJ-RQ4587
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4587-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LUM antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) placenta, 2) Caco-2, 3) CCRF-CEM, 4) HeLa and 5) Jurkat lysate with LUM antibody at 0.5ug/ml. Expected moleculer weight: ~40 kDa (unmodified), ~60 kDa (glycosylated).
2 / 6
Western blot testing of mouse 1) liver, 2) ovary, 3) testis, 4) lung and rat 5) liver, 6) ovary, 7) testis, 8) lung and 9) heart lysate with LUM antibody at 0.5ug/ml. Expected moleculer weight: ~40 kDa (unmodified), ~60 kDa (glycosylated).
3 / 6
IHC staining of FFPE human placenta with LUM antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 6
IHC staining of FFPE human ovarian cancer with LUM antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
IHC staining of FFPE human pancreatic cancer with LUM antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 6
IHC staining of FFPE human lung cancer with LUM antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of human 1) placenta, 2) Caco-2, 3) CCRF-CEM, 4) HeLa and 5) Jurkat lysate with LUM antibody at 0.5ug/ml. Expected moleculer weight: ~40 kDa (unmodified), ~60 kDa (glycosylated).
Western blot testing of mouse 1) liver, 2) ovary, 3) testis, 4) lung and rat 5) liver, 6) ovary, 7) testis, 8) lung and 9) heart lysate with LUM antibody at 0.5ug/ml. Expected moleculer weight: ~40 kDa (unmodified), ~60 kDa (glycosylated).
IHC staining of FFPE human placenta with LUM antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human ovarian cancer with LUM antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human pancreatic cancer with LUM antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human lung cancer with LUM antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein)
Application Note:
Optimal dilution of the LUM antibody should be determined by the researcher.
Description:
Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. Lumican is a major keratan sulfate proteoglycan of the cornea but is ubiquitously distributed in most mesenchymal tissues throughout the body. And Lumican is involved in collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Corneal transparency is possible due to the exact alignment of collagen fibers by lumican (and keratocan) in the intrafibrillar space.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids A50-N338 from the human protein were used as the immunogen for the LUM antibody.
Limitation:
This LUM antibody is available for research use only.
Localization:
Secreted, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P51884