TNF Receptor I Antibody / TNFR1 / TNFRSF1A

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NSJ Bioreagents
Product Code: NSJ-RQ4614
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4614-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TNF Receptor antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE mouse lung with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 5
IHC staining of FFPE mouse liver with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 5
IHC staining of FFPE mouse kidney with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 5
Flow cytometry testing of human U937 cells with TNF Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TNF Receptor antibody.
5 / 5
Western blot testing of 1) human HeLa, 2) human K562, 3) human Caco-2, 4) rat liver, 5) mouse small intestine, 6) mouse liver and 7) mouse HEPA1-6 lysate with TNF Receptor antibody at 0.5ug/ml. Predicted molecular weight ~51 kDa.
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IHC staining of FFPE mouse lung with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse liver with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse kidney with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human U937 cells with TNF Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TNF Receptor antibody.
Western blot testing of 1) human HeLa, 2) human K562, 3) human Caco-2, 4) rat liver, 5) mouse small intestine, 6) mouse liver and 7) mouse HEPA1-6 lysate with TNF Receptor antibody at 0.5ug/ml. Predicted molecular weight ~51 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,ELISA (Capture): 1-5ug/ml (recombinant human protein)
Application Note:
Optimal dilution of the TNF Receptor antibody should be determined by the researcher.
Description:
Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), also known as TNFR1, is a protein that in humans is encoded by the TNFRSF1A gene. The protein encoded by this gene is a member of the Tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. The TNFR1 gene is mapped to 12pter-cen. It encodes a protein of 455 amino acids. And this receptor can activate the transcription factor NF-?B, mediate apoptosis, and function as a regulator of inflammation.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids I22-T211 from the human protein were used as the immunogen for the TNF Receptor antibody.
Limitation:
This TNF Receptor antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P19438