Thioredoxin Antibody / TRX / TXN

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NSJ Bioreagents
Product Code: NSJ-RQ4619
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4619-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TRX antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of mouse 1) testis, 2) kidney, 3) small intestine, 4) spleen, 5) stomach, 6) lung, 7) smooth muscle and 8) heart lysate with TXN antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.
2 / 6
Western blot testing of rat 1) testis, 2) kidney, 3) small intestine, 4) spleen, 5) stomach, 6) lung, 7) smooth muscle and 8) heart lysate with TXN antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.
3 / 6
IHC staining of FFPE mouse small intestine with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 6
ICC staining of FFPE mouse LLC cells with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
IHC staining of FFPE rat spleen with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 6
Immunofluorescent staining of mouse NIH3T3 cells with TRX antibody (green, top left) and DAPI nuclear stain (blue, top right), and overlay (bottom).
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Western blot testing of mouse 1) testis, 2) kidney, 3) small intestine, 4) spleen, 5) stomach, 6) lung, 7) smooth muscle and 8) heart lysate with TXN antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.
Western blot testing of rat 1) testis, 2) kidney, 3) small intestine, 4) spleen, 5) stomach, 6) lung, 7) smooth muscle and 8) heart lysate with TXN antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.
IHC staining of FFPE mouse small intestine with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
ICC staining of FFPE mouse LLC cells with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat spleen with TRX antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of mouse NIH3T3 cells with TRX antibody (green, top left) and DAPI nuclear stain (blue, top right), and overlay (bottom).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immuocytochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Direct ELISA: 0.1-0.5ug/ml (mouse recombinant protein)
Application Note:
Optimal dilution of the TRX antibody should be determined by the researcher.
Description:
Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids V2-A105 from the mouse protein were used as the immunogen for the TRX antibody.
Limitation:
This TRX antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P10639