ADA Antibody / Adenosine deaminase

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NSJ Bioreagents
Product Code: NSJ-RQ4623
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4623-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 6D4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ADA antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) HeLa, 2) placenta, 3) A549, 4) MCF7, 5) U-937, 6) U-2 OS and 7) Caco-2 lysate with ADA antibody at 0.5ug/ml. Predicted molecular weight ~41 kDa.
2 / 6
IHC staining of FFPE human colon cancer with ADA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 6
IHC staining of FFPE human colon cancer with ADA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 6
IHC staining of FFPE human rectal cancer with ADA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
IHC staining of FFPE human tonsil with ADA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 6
Flow cytometry testing of human U-2 OS cells with ADA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADA antibody.
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Western blot testing of human 1) HeLa, 2) placenta, 3) A549, 4) MCF7, 5) U-937, 6) U-2 OS and 7) Caco-2 lysate with ADA antibody at 0.5ug/ml. Predicted molecular weight ~41 kDa.
IHC staining of FFPE human colon cancer with ADA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human colon cancer with ADA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human rectal cancer with ADA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human tonsil with ADA antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human U-2 OS cells with ADA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADA antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the ADA antibody should be determined by the researcher.
Description:
Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Q135-L363 from the human protein were used as the immunogen for the ADA antibody.
Limitation:
This ADA antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P00813