AKT2 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4627
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4627-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 10C6
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the AKT2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with AKT2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human A549 cells with AKT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKT2 antibody.
3 / 3
Western blot testing of human 1) A549, 2) 293T, 3) HeLa, 4) Caco-2, 5) K562, 6) HL60 and 7) PC-3 lysate with AKT2 antibody at 0.5ug/ml. Predicted molecular weight ~56 kDa.
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Immunofluorescent staining of FFPE human MCF7 cells with AKT2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with AKT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKT2 antibody.
Western blot testing of human 1) A549, 2) 293T, 3) HeLa, 4) Caco-2, 5) K562, 6) HL60 and 7) PC-3 lysate with AKT2 antibody at 0.5ug/ml. Predicted molecular weight ~56 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the AKT2 antibody should be determined by the researcher.
Description:
AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. This gene is mapped to 19q13.2. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids DRYDSLGLLELDQRTHFPQFSYSASIRE were used as the immunogen for the AKT2 antibody.
Limitation:
This AKT2 antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P31751