Anillin Antibody / Scraps

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NSJ Bioreagents
Product Code: NSJ-RQ4636
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4636-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
After reconstitution, the Anillin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) placenta, 2) SW620, 3) A431, 4) HL60, 5) PC-3 and 6) mouse NIH 3T3 lysate with Anillin antibody at 0.5ug/ml. Predicted molecular weight ~124 kDa.
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Flow cytometry testing of human U-2 OS cells with Anillin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Anillin antibody.
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Western blot testing of human 1) placenta, 2) SW620, 3) A431, 4) HL60, 5) PC-3 and 6) mouse NIH 3T3 lysate with Anillin antibody at 0.5ug/ml. Predicted molecular weight ~124 kDa.
Flow cytometry testing of human U-2 OS cells with Anillin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Anillin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Anillin antibody should be determined by the researcher.
Description:
Anillin is a conserved protein implicated in cytoskeletal dynamics during cellularization and cytokinesis. This gene is mapped to 7p14.2. The ANLN gene in humans and the scraps gene in Drosophila encode Anillin. The human anillin cDNA, located on Chr7, encodes a 1,125-amino acid protein with a predicted molecular mass of 124 kD and a pI of 8.1. The mouse anillin gene is located on Chr9. This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KEERDLWMQKLNQVLVDIRLWQPDACYK were used as the immunogen for the Anillin antibody.
Limitation:
This Anillin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9NQW6