Bcl6 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4637
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4637-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Bcl6 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of human 1) Raji, 2) MCF7, 3) placenta, 4) A549, 5) Caco-2 and 6) U-2 OS lysate with Bcl6 antibody at 0.5ug/ml. Predicted molecular weight: 78~100 kDa.
2 / 4
Western blot testing of rat 1) testis, 2) spleen, 3) brain and mouse 4) testis, 5) spleen and 6) brain lysate with Bcl6 antibody at 0.5ug/ml. Predicted molecular weight: 78~100 kDa.
3 / 4
IHC staining of FFPE mouse spleen tissue with Bcl6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 4
Flow cytometry testing of rat BRL 3A cells with Bcl6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bcl6 antibody.
❮❯

Western blot testing of human 1) Raji, 2) MCF7, 3) placenta, 4) A549, 5) Caco-2 and 6) U-2 OS lysate with Bcl6 antibody at 0.5ug/ml. Predicted molecular weight: 78~100 kDa.
Western blot testing of rat 1) testis, 2) spleen, 3) brain and mouse 4) testis, 5) spleen and 6) brain lysate with Bcl6 antibody at 0.5ug/ml. Predicted molecular weight: 78~100 kDa.
IHC staining of FFPE mouse spleen tissue with Bcl6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of rat BRL 3A cells with Bcl6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bcl6 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Bcl6 antibody should be determined by the researcher.
Description:
B-cell lymphoma 6 protein is a protein that in human is encoded by the BCL6 gene. Like BCL2, BCL3, BCL5, BCL7A, BCL9 and BCL10, it has clinical significance in lymphoma. The protein encoded by this gene is an evolutionarily conserved zinc finger transcription factor and contains an N-terminal POZ/BTB domain. This protein acts as a sequence-specific repressor of transcription and has been shown to modulate the STAT-dependent Interleukin 4 (IL-4) responses of B cells. This protein can interact with several corepressor complexes to inhibit transcription. This gene is found to be frequently translocated and hypermutated in diffuse large B cell lymphoma (DLBCL) and contributes to the pathogenesis of DLBCL. An exon 7 skipping splice variant encodes a shorter form of the protein which lacks the first two zinc fingers of the DNA binding domain.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ASPADSCIQFTRHASDVLLNLNRLRSRDILTD were used as the immunogen for the Bcl6 antibody.
Limitation:
This Bcl6 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P41182