uPAR Antibody / uPA Receptor

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NSJ Bioreagents
Product Code: NSJ-RQ4654
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4654-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the uPAR antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) placenta, 2) U-2 OS, 3) A431, 4) HeLa and 5) A549 lysate with uPAR antibody at 0.5ug/ml. Expected molecular weight: 37-60 kDa, depending on glycosylation level.
2 / 3
Western blot testing of 1) rat testis, 2) mouse small intestine, 3) mouse kidney and 4) mouse testis lysate with uPAR antibody at 0.5ug/ml. Expected molecular weight: 37-60 kDa, depending on glycosylation level.
3 / 3
Flow cytometry testing of human PBM with uPAR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= uPAR antibody.
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Western blot testing of human 1) placenta, 2) U-2 OS, 3) A431, 4) HeLa and 5) A549 lysate with uPAR antibody at 0.5ug/ml. Expected molecular weight: 37-60 kDa, depending on glycosylation level.
Western blot testing of 1) rat testis, 2) mouse small intestine, 3) mouse kidney and 4) mouse testis lysate with uPAR antibody at 0.5ug/ml. Expected molecular weight: 37-60 kDa, depending on glycosylation level.
Flow cytometry testing of human PBM with uPAR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= uPAR antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the uPAR antibody should be determined by the researcher.
Description:
PLAUR (PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. PLAUR is originally identified as a saturable binding site for urokinase on the cell surface. And the gene plays an important role in many normal as well as pathologic processes. The PLAUR gene is localized to 19q13.31. PLAUR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. PLAUR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus it seems to be an important player in the regulation of this process. In human coronary artery vascular smooth muscle cells, UPA stimulates cell migration via a UPAR signaling complex containing TYK2 and phosphatidylinositol 3-kinase.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids TKSGCNHPDLDVQYRS were used as the immunogen for the uPAR antibody.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q03405