METTL3 Antibody / M6A

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NSJ Bioreagents
Product Code: NSJ-RQ4669
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4669-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the METTL3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of mouse HEPA1-6 cells with METTL3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= METTL3 antibody.
2 / 2
Western blot testing of 1) human HepG2, 2) human PC-3, 3) human A549, 4) human HaCaT, 5) rat brain, 6) rat testis and 7) mouse brain lysate with METTL3 antibody at 0.5ug/ml. Expected molecular weight: 64-70 kDa.
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Flow cytometry testing of mouse HEPA1-6 cells with METTL3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= METTL3 antibody.
Western blot testing of 1) human HepG2, 2) human PC-3, 3) human A549, 4) human HaCaT, 5) rat brain, 6) rat testis and 7) mouse brain lysate with METTL3 antibody at 0.5ug/ml. Expected molecular weight: 64-70 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the METTL3 antibody should be determined by the researcher.
Description:
N6-adenosine-methyltransferase 70 kDa subunit (METTL3) is an enzyme that in humans is encoded by the METTL3 gene. It is mapped to 14q11.2. This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Q423-D571 from the human protein were used as the immunogen for the METTL3 antibody.
Limitation:
This METTL3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q86U44