Interferon gamma Antibody / IFNG

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NSJ Bioreagents
Product Code: NSJ-RQ4575
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4575-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IFNG antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) HeLa, 2) placenta, 3) U-87 MG, 4) HL-60 and 5) U-937 lysate with IFNG antibody at 0.5ug/ml. Predicted molecular weight ~19 kDa.
2 / 6
Western blot testing of 1) rat brain, 2) rat lung, 3) mouse brain, 4) mouse ovary and 5) mouse HEPA 1-6 lysate with IFNG antibody at 0.5ug/ml. Predicted molecular weight ~19 kDa.
3 / 6
IHC staining of FFPE human sarcoma with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human lung cancer with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE human lung cancer with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 6
IHC staining of FFPE human tonsil tissue with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human 1) HeLa, 2) placenta, 3) U-87 MG, 4) HL-60 and 5) U-937 lysate with IFNG antibody at 0.5ug/ml. Predicted molecular weight ~19 kDa.
Western blot testing of 1) rat brain, 2) rat lung, 3) mouse brain, 4) mouse ovary and 5) mouse HEPA 1-6 lysate with IFNG antibody at 0.5ug/ml. Predicted molecular weight ~19 kDa.
IHC staining of FFPE human sarcoma with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA (Capture): 1-5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the IFNG antibody should be determined by the researcher.
Description:
IInterferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Q24-G161 from the human protein were used as the immunogen for the IFNG antibody.
Limitation:
This IFNG antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P01579