Smooth Muscle Actin Antibody [SPM332]
| Code | Size | Price |
|---|
| PSI-34-162-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM332
Regulatory Status: RUO
Target Species:
- Baboon
- Cavia (Guinea pig)
- Chicken (Gallus)
- Feline (cat)
- Human
- Monkey
- Mouse
- Rabbit
- Rat
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin, ACTVS, GIG46
Application Note:
Western blot: 1-2 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The optimal dilution of the Smooth Muscle Actin antibody for each application should be determined by the researcher.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The optimal dilution of the Smooth Muscle Actin antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
Actin is a major component of the cytoskeleton and is present in most cell types. This mAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A 10 amino acid peptide from the alpha smooth muscle isoform of actin was used as the immunogen for this Smooth Muscle Actin antibody.
NCBI Gene ID #:
59
NCBI Official Name:
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol:
ACTA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P62736
User NOte:
Optimal dilutions for each application to be determined by the researcher