MCM7 Antibody [MCM7/1466]
| Code | Size | Price |
|---|
| PSI-33-725-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: MCM7/1466
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2
Application Note:
IF: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Background:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 195-319 were used as the immunogen for the MCM7 antibody.
NCBI Gene ID #:
4176
NCBI Official Name:
minichromosome maintenance complex component 7
NCBI Official Symbol:
MCM7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
P33993
User NOte:
Optimal dilutions for each application to be determined by the researcher