ALDH1 Antibody [AHDH1-1]

ProSci
Product Code: PSI-34-040
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-34-040-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: AHDH1-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

1 / 2
IHC testing of FFPE human colon carcinoma with ALDH1 antibody (clone AHDH1-1).
2 / 2

IHC testing of FFPE human colon carcinoma with ALDH1 antibody (clone AHDH1-1).

Further Information

Additional Names:
Retinal dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, ALDH1A1, ALDC, ALDH1, PUMB1
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells

Western blot: 1-2 ug/ml

IF: 1-2 ug/ml

IHC (FFPE): 0.5-1 ug/ml

Titering of the ALDH1 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Background:
ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant ALDH1A1 protein (AA 315-434) was used as the immunogen for this ALDH1 antibody.
NCBI Gene ID #:
216
NCBI Official Name:
aldehyde dehydrogenase 1 family, member A1
NCBI Official Symbol:
ALDH1A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P00352
User NOte:
Optimal dilutions for each application to be determined by the researcher