Biotin Antibody [SB58c] (HRP)

ProSci
Product Code: PSI-99-182
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-99-182-1mL1mL£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: [SB58c]
Regulatory Status: RUO
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Store vial at 2-8˚C

Images

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Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (Cat. No. 99-182) secondary antibody and chemiluminescent detection.

Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (Cat. No. 99-182) secondary antibody and chemiluminescent detection.

Further Information

Application Note:
Biotin Antibody [SB58c] for use in ELISA and western blotting assays.
Background:
Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives.
Buffer:
Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Concentration:
Lot specific
Conjugate:
HRP
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Biotin-KLH
Physical State:
Liquid
Research Area:
Obesity,Signal Transduction
SPECIFICITY:
Biotin
User NOte:
Optimal dilutions for each application to be determined by the researcher.