Bcl6 Antibody [BCL6/1526]
| Code | Size | Price |
|---|
| PSI-33-802-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: BCL6/1526
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
BCL6 Antibody: BCL5, LAZ3, BCL6A, ZNF51, ZBTB27, BCL5, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, BCL-6
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the Bcl6 antibody should be determined by the researcher.
Immunofluorescence: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the Bcl6 antibody should be determined by the researcher.
Background:
Antibody to Bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show Bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, Bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 256-389 from the human protein were used as the immunogen for the Bcl6 antibody.
NCBI Gene ID #:
604
NCBI Official Name:
B-cell CLL/lymphoma 6
NCBI Official Symbol:
BCL6
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle
Swissprot #:
P41182
User NOte:
Optimal dilutions for each application to be determined by the researcher